Annu. Trends in Cell Biol. Microinjection is a direct method to introduce DNA into either cytoplasm or nucleus. Deom C M, Schubert K, Wolf S, Holt C, Lucas W J and Beachy R N 1990 Molecular characterization and biological function of the movement protein of tobacco mosaic virus in transgenic plants. This has the potential to expand the application of microinjection to other procedures, such as production of over-expressed proteins for western blot, immunoprecipitation, purification, and bioluminescence, where they are critically needed but cannot be implemented due to inherent drawbacks of … Kochian L V and Lucas W J 1988 Potassium transport in roots. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence: Amazon.sg: Books Bush D R 1993 Proton-coupled sugar and amino acid transporters in plants. (gross), © 2020 Springer Nature Switzerland AG. Applications. Current Opinion Cell Biol. 100, 1433–1441. Plant Cell Physiol. Waigmann E, Lucas W J, Citovsky V and Zambryski P 1994 Direct functional assay for tobacco mosaic virus cell-to-cell movement protein and identification of a domain involved in increasing plasmodesmal permeability. Phytopathol. 1. Polymer Microinjection Molding Market Types, Regions and Applications Research Report Forecast to 2027 : Accu-Mold LLC, ALC Precision, Stamm AG, Sovrin Plastics, Etc. common for animal cells. ...you'll find more products in the shopping cart. Fabrication of a cup-shaped microforceps beak-half using a microforge. Hake S and Freeling M 1986 Analysis of genetic mosaics shows that the extra epidermal cell divisions in. Ding B, Haudenshield J S, Hull R J, Wolf S, Beachy R N and Lucas W J 1992 Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants. Spanswick R M and Costerton J W F 1967 Plasmodesmata in. Plant Cell Physiol. 79, 771–776. Plant Mol. 114, 475–481. Plant Physiol. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence. Kochian L V, Xin-Zhi J and Lucas W J 1985 Potassium transport in corn roots. It is a microsurgical procedure conducted on a single cell, using a glass needle (i.e., a fine, glass microcapillary pipette), a precision positioning device (a micromanipulator) to control the movement of the micropipette, and a microinjector. Microinjection is frequently used in genetic engineering andtransgenetics to insert genetic material into single cells. Rev. Bostwick D E, Dannenhoffer J M, Skaggs M I, Lister R M, Larkins B A and Thompson GA 1992 Pumpkin phloem lectin genes are specifically expressed in companion cells. 70, 1723–1731. Producing a microinjection needle requires experience and careful handling. A borosilicate glass capillary is heated and drawn out, then bent using forceps to produce a needle tip of around 10-30 micrometers. Carroll, David J. Ishiwatari, Y, Honda C, Kawashima I, Nakamura S, Hirano H, Mori S, Fujiwara T, Hayashi H and Chino M 1995 Thioredoxin h is one of the major proteins in rice phloem sap. However, factors … Rev. Adv. 4, 189–195. The working distance of the condenser, measured from the lowest part of the condenser to the stage, is very important. Ding B, Haudenshield J S, Willmitzer L and Lucas W J 1993 Development of secondary plasmodesmata is arrested in tobacco expressing a yeast derived invertase gene. Natl. Resolution of kinetics into a saturable and a linear component. 4, 179–189. Olesinski A A, Lucas W J, Galun E and Wolf S 1995 Pleiotropic effects of TMV-MP on carbon metabolism and export in transgenic tobacco plants. Plant Cell Environ. 47, 595–626. … I would like all the readers … to get a copy of the book: I am sure they will broad the paraphernalia usefull to carry on succesfull researches." Plant Cell Environ. 7, 673–680. USA 91, 1433–1437. Nakamura S, Hayashi H, Mori S and Chino M 1996 Detection and characterization of protein kinases in rice phloem sap. Sci. The application of microinjection methods, in conjunction with molecular tools, established. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. 45, 211–234. Springer-Verlag, Berlin, Germany. pp 25-39 | Microinjectors For All Your Microinjection Applications Designed to simplify intracellular injection and a variety of other microinjection tasks, the WPI introduces three new Microinjectors: Type MICRO-ePUMP uPUMP PV850 Regulated compensation and injection pressure Yes Yes Yes Pressure output: 0.3–87 PSI Yes … Proc. Sci. Plant Physiol. Sommer H, Beltran JP, Huijser P, Pape H, Lonnig W E, Saedler H and Schwartz-Sommer Z 1990. Acad. Wolf S and Lucas W J 1997 Plasmodesmal-mediated plant communication network: Implications for controlling carbon metabolism and resource allocation. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence: Bach, P H, European Workshop on … Besides providing insight into the molecular nature of many of the membrane transport systems that function in nutrient acquisition and transport, this approach revealed the presence of a unique plasmodesmal macromolecular trafficking system that operates at the cellular/tissue and whole-plant level. 12, 49–52. Plant Mol. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Part of Springer Nature. Nissen P 1974 Uptake mechanisms: inorganic and organic. 32, 251–273. 32, 387–411. The application of microinjection methods, in conjunction with molecular tools, established a powerful experimental approach to elucidate the processes underlying plant growth and development. http://experiment.worldcat.org/entity/work/data/330043#Topic/molecular_probe_techniques_congresses; http://id.worldcat.org/fast/832759 Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research. 44, 513–542. 21, 1319–1327. Olesinski A A, Almon E, Navot N, Galun E, Lucas W J and Wolf S 1996 Tissue specific expression of tobacco mosaic virus movement protein in transgenic potato plants alters plasmodesmal function and carbohydrate partitioning. (CarloAlberto Redi, European Journal of Histochemistry, October, 2009), Expression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation, Use of Luciferase Chimaera to Monitor PLCζ Expression in Mouse Eggs, Analysis of 14-3-3 Family Member Function in Xenopus Embryos by Microinjection of Antisense Morpholino Oligos, A Microinjectable Biological System, the Xenopus Oocyte, as an Approach to Understanding Signal Transduction Protein Function, Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs, Analysis of Signaling Pathways in Zebrafish Development by Microinjection, Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complementary Approaches to Study Protein Function, DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cell Lines, Bacteriophage φC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels, Germline Transformation of Caenorhabditis elegans by Injection, Quantitative Microinjection of Mouse Oocytes and Eggs, Microinjection of Follicle-Enclosed Mouse Oocytes, Functional Studies of Regulatory Genes in the Sea Urchin Embryo, Exploring the Cytoskeleton During Intracytoplasmic Sperm Injection in Humans, Somatic Cell Nuclear Transfer in the Mouse. Münch E 1930 Die Stoffbewegung in der Pflanze. Plant Molec. The Eppendorf electronic microinjectors, the FemtoJet ® 4i and FemtoJet 4x, are your tools of choice for reproducibly injecting small volumes into adherent cells and suspension cells. Kochian L V and Lucas W J 1982a Potassium transport in corn rrots. Plant Physiol. a powerful experimental approach to elucidate the processes underlying plant growth and development. The significance of the root periphery. Vorob’ev L N, Tarkhanov K A and Vakhmistrov D B 1982 Use of the electrical coupling factor for quantitative estimation of symplasmic communications. Planta 102, 215–227. Lucas W J, Balachandran S, Park J and Wolf S 1996 Piasmodesmal companion cell-mesophyll communication in the control over carbon metabolism and phloem transport: insights gained from viral movement proteins. Sci. a A flow of air is blown from the left, and the tip of a 1 ~ micropipette is closed by approaching it with the wann filament b. Application of microinjection techniques to plant nutrition Application of microinjection techniques to plant nutrition Lucas, William 1997-10-01 00:00:00 To gain a full understanding of the complex processes that underlie plant nutrition requires the elucidation of the genetic, molecular, biochemical, biophysical, … Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Sakuth T, Schobert C, Pecsvaradi A, Eichholz A, Komor E and Orlich G 1993 Specific proteins in the sieve-tube exudates of, Schachtman D P, Schroeder J I, Lucas W J, Anderson J A and Gaber RF 1992 Expression of an inward-rectifying potassium channel by the, Schobert C, Großmann P, Gottschalk M, Komor E, Pecsvaradi A and Nieden U Z 1995 Sieve-tube exudate from. Plant Mol. Lucas W J, Olesinski A, Hull R J, Haudenshield J, Deom C M, Beachy R N and Wolf S 1993b Influence of the tobacco mosaic virus 30-kDa movement protein on carbon metabolism and photosynthate partitioning in transgenic tobacco plants. Kawabe S, Fukumorita T and Chino M 1980 Collection of rice phloem sap from stylets of homopterous insects severed by YAG laser. Karrer E E, Lincoln J E, Hogenhout S, Bennett A B, Bostock R M, Martineau B, Lucas W J, Gilchrist D G and Alexander D 1995 In situ isolation of mRNA from individual cells: creation of cell-specific cDNA libraries. 18, 1301–1310. Future studies aimed at identifying additional genes associated with this plasmodesmal macromolecular trafficking system will advance our understanding of the function and evolution of this novel plant communication system. As example for a knock-out approach, we address the loss of the oculocutaneous albinism 2 (oca2, Lischik et al., 2019) Jackson D, Veit B and Hake S 1994 Expression of maize. Lucas W J and Wolf S 1993 Plasmodesmata: the intercellular organelle of green plants. Plant Physiol. Natl. Lucas W J and Gilbertson R L 1994 Plasmodesmata in relation to viral movement within leaf tissues. Detailed protocols for a wide range of applications … USA 92, 3814–3818. dextran - fluorescein isothyocyanate conjugated dextran. Microinjection processes use either metal microinjection needles or glass micropipettes to inject small liquid volumes. Biol. 114, 305–340. Trends Biochem. Science 256, 663–665. Cite as. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. 28, 495–502. Cleland R E, Fujiwara T and Lucas W J 1994 Plasmodesmal-mediated cell-to-cell transport in wheat roots is modulated by anaerobic stress. One end of a glass micropipette is heated until the glass becomes somewhat liquified. Bot. Wolf S, Deom C M, Beachy R and Lucas W J 1991 Plasmodesmatal function is probed using transgenic tobacco plants that express a virus movement protein. ... April 1992, cell biologists explore how the microinjection of defined material can be used to manipulate the fundamental biochemistry of specific living cells. Reynolds, J.M. Plant Cell Physiol. S.No. This is a preview of subscription content, Anderson J A, Huprikar S S, Kohian L V, Lucas W J and Gaber R F 1992 Functional expression of a probably. Microinjection can also be used in basic genetics research, in thecloning of organisms, and in the study of cell biology and viruses. The application of microinjection methods, in conjunction with molecular tools, established a powerful experimental approach to elucidate the processes underlying plant growth and development. Biol. This information processing network it discussed in terms of its role in allowing plants to regulate physiological activities at a supracellular level. http://schema.org/about. Characterization of the linear component. Table 7: Applications of microinjection. 387p. Over 10 million scientific documents at your fingertips. Annu. Carbon dioxide gas at 1.2 bar is pressed into the pipette, blowing the melted tip of the pipette into a sphere. Expression of exogenous mRNA in Xenopus laevis embryos for the study of cell cycle regulation / Jill C. Sible and Brian N. Wroble --Use of luciferase chimaera to monitor PLC[zeta] expression in mouse eggs / Karl Swann [and others] --Analysis of 14-3-3 family member function in Xenopus embryos by microinjection of antisense … © 2020 Springer Nature Switzerland AG. Ishiwatari Y, Fujiwara T, McFarland K C, Nemoto K, Hayashi H, Chino M and Lucas W J 1997 Rice phloem thioredoxin h has the capacity to mediate its own cell-to-cell transport through plasmodesmata. Soviet Plant Physiol. Overall R L, and Gunning B E S 1982 Intercellular communication in. This video has introduced the microinjection setup, demonstrated how to prepare microinjection needles, shown how to prepare embryos for microinjection, perform the microinjection technique, and some applications of microinjection. Plant Cell 4, 1539–1548. Happy Holidays—Our $/£/€30 Gift Card just for you, and books ship free! Libros Los más vendidos Infantil y Juvenil Literatura y Ficción Libros de texto Negocios e Inversiones Tienda de Comics Tienda Kindle Los más vendidos Infantil y … This Application Note details our microinjection workflow for CRISPR/Cas9 mediated knock-out and knock-in approaches using the Eppendorf microinjection system. providing insight into the molecular nature of many of the membrane transport systems that function in … Gustav Fischer, Jena. Microinjection. Planta 195, 456–463. Tester M 1990 Plant ion channels: whole-cell and single-channel studies. Hedrich R and Schroeder J I 1989 The physiology of ion channels and electrogenic pumps in higher plants. Annu. Plant Physiol. Process is applicable for plant cell as well as animal cell but more . Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence: Proceedings of the First European Workshop Held in … DNA microinjection methods are used to transfer genes between animals and are a popular technique for creating transgenic organisms, particularly mammals. Plant Cell 3, 593–604. Mezitt L A, and Lucas W J 1996 Plasmodesmal cell-to-cell transport of proteins and nucleic acids. 41, 369–419. To gain a full understanding of the complex processes that underlie plant nutrition requires the elucidation of the genetic, molecular, biochemical, biophysical, physiological and environmental factors that interact, at the cellular, organ and whole plant levels, to allow this sessile organism to optimize the allocation and utilization of available resources. "Chapters cover all of the hot topics in which the technique can be used (namely, all the research field you are involved!) I. 34, 927–933. In cases where very small volumes need to be injected (less than 2 nanoliters), gas-pressure regulated microinjectors may be used. Spanswick R M 1972 Electrical coupling between cells of higher plants: a direct demonstration of intercellular communication. Goodwin P B 1983 Molecular size limit for movement in the symplast of the, Goodwin P B and Lyndon R F 1983 Synchronisation of cell division during transition to flowering in. JavaScript is currently disabled, this site works much better if you Kochian L V and Lucas W J 1982b A re-evaluation of the carrier-kinetic approach to ion transport in roots of higher plants. New Phytol. We have a dedicated site for United Kingdom. Fisher D B, Wu Y and Ku M S B 1992 Turnover of soluble proteins in the wheat sieve tube. Proc. Wolf S, Deom C M, Beachy R N and Lucas W J 1989 Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit. Robards A W and Lucas W J 1990 Plasmodesmata. Especially in the field of male infertility, this groundbreaking application is widely used and the suspicion that anomalies will occur in the pregnancies that occur despite the birth of thousands of babies all over the world … Balachandran S, Hull R J, Vaadia Y, Wolf S and Lucas W J 1995 Tobacco mosaic virus movement protein-induced change in carbon partitioning originates from the mesophyll and is independent of change in plasmodesnial size exclusion limit. 3, 308–315. Plant Physiol. Science 270, 1980–1983. Bot. Plant Physiol. C. H. Reynolds. Microinjection can also be used in the cloning of organisms, in the study of cell biology and viruses, and for treating male subfertility through intracytoplasmic sperm injection (ICSI, / ˈ ɪ k s i / IK-see Science 275, 1298–1300. Tanner W and Caspari T 1996 Membrane transport carriers. Plant Physiol. Frommer W B Hunnel S and Riesmeier J W 1993 Expression cloning in yeast of a cDNA encoding a broad specificity amino acid permease from. J. Exp. Borstlap A C 1981 Invalidity of the multiphasic concept of ion absorption in plants. II. 40, 539–569. Typically, microinjection is performed under a microscope. Editors: and so many different model systems … associated with other master technique (e.g., western blot analysis). Not logged in Rev. AREAS OF APPLICATION AND USE. Trobner W, Ramirez L, Motte P, Hue I, Huijser P, Lonnig W E, Saedler H, Sommer H and Schwartz-Sommer Z 1992. Balachandran S, Hull R J, Martins R A, Vaadia Y and Lucas W J 1997a Influence of environmental stress on biomass partitioning in transgenic tobacco plants expressing the movement protein of tobacco mosaic virus. Sussman M R 1994 Molecular analysis of proteins in the plasma membrane. (Ed.). Besides. Biol. Plant J. Planta 190, 88–96. Plant Physiol. Download preview PDF. … the beginners can immediately get good self-trainings tricks that will ensure them to reach good results. Unable to display preview. Microinjection Microinjection is a technique of delivering foreign DNA into a living cell (a cell, egg, oocyte, embryos of animals) through a glass micropipette. Rev. The microinjection polymer molding market growth is driven by demand for micro gears, replacing affordable feedstock like PEEK. Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions. Plant Physiol. Annu. USA 87, 3284–3288. Sci. 125, 435–476. Erwee M G and Goodwin P B 1983 Characterisation of the, Erwee M G and Goodwin P B 1984 Characterization of the, Erwee M G and Goodwin P B 1985 Symplast domains in extrastellar tissues of, Erwee M G, Goodwin P B and Van Bel A J E 1985 Cell-cell communication in the leaves of. Plant Cell 4, 915–928. Science 237, 384–389. Springer is part of, Please be advised Covid-19 shipping restrictions apply. Proc. You’ve just watched JoVE’s introduction to microinjection of early zebrafish embryos. Cell 76, 925–932. Fujiwara T, Giesman-Cookmeyer D, Ding B, Lommel S A and Lucas W J 1993 Cell-to-cell trafficking of macromolecules through plasmodesmata potentiated by the red clover necrotic mosaic virus movement protein. Lucas W J, Ding B and van der Schoot C 1993a Plasmodesmata and the supracellular nature of plants. Plant Nutrition for Sustainable Food Production and Environment, Section of Plant Biology, Division of Biological Sciences, https://doi.org/10.1007/978-94-009-0047-9_3. Res. 15, 93–178. Lucas W J, Bouche-Pillon S, Jackson D P, Nguyen L, Baker L, Ding B, and Hake S 1995 Selective trafficking of KNOTTED1 and its mRNA through plant plasmodesmata. Not affiliated Nakamura S, Hayashi H, Mori S and Chino M 1993 Protein phosphorylation in the sieve tubes of rice plants. 111, 541–550. An Explanation of DNA DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence: Peter H. Bach, C.H. Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. Deom C M, Oliver M J, and Beachy R N 1987 The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. 86.107.224.179. Since the first application in 1992, there has been rapid development in the field of microinjection. Planta 197, 118–126. Lucas W J 1995 Plasmodesmata: intercellular channels for macromolecular transport in plants. Sentenac H, Bonneaud N, Minet M, Lacroute F, Salmon J-M, Gaymard F and Grignon C. 1992 Cloning and expression in yeast of a plant potassium transport system. Rev. This service is more advanced with JavaScript available, Plant Nutrition for Sustainable Food Production and Environment Protoplasma 178, 81–85. Noueiry A O, Lucas W J and Gilbertson R L 1994 Two Proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. It seems that you're in United Kingdom. Kühn C, Franceschi V R, Schulz A, Lemoine R and Frommer W B 1997 Macromolecular trafficking indicated by localization and turnover of sucrose transporters in enucleate sieve elements. What’s New In Plant Physiol. Balachandran S, Xiang Y, Schobert C, Thompson G A and Lucas W J 1997b Phloem sap proteins of, Behrens H M, Gradmann D and Sievers A 1985 Membranepotential responses following gravistimulation in roots of. Planta (In Press). This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Please review prior to ordering, Explores a wide variety of model systems, including human, mouse, sea urchin, starfish, zebrafish, and Xenopus oocytes, transformed Caenorhabditis elegans, and tissue culture cells, Contains detailed methods easily adaptable to virtually any system, Covers gene and protein function in living cells, analysis of signal transduction pathways in vivo, single cell analysis, systems biology in a living cell, quantitative analysis of signaling transduction, antisense morpholino oligonucleotide and RNAi analysis, quantitative microinjection, and analysis of gene expression, among other topics, Expands the capabilities of cellular and molecular biology laboratories and provides scientists with a valuable mechanistic understanding, ebooks can be used on all reading devices, Institutional customers should get in touch with their account manager, Usually ready to be dispatched within 3 to 5 business days, if in stock, The final prices may differ from the prices shown due to specifics of VAT rules. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence Edited by P. H. Bach published on October, 2012: Amazon.es: Libros Rev. In combination with the TransferMan ® 4r or InjectMan ® 4 they form an ideal … c. The … Figure 8. 25, 53–79. The application of microinjection methods, in conjunction with molecular tools, established a powerful experimental approach to elucidate the processes underlying plant growth and development. IV. Plant Cell 5, 1783–1794. price for Spain Shop now! 2. Chilcott T C, Frost-Shartzer S, Lverson M W, Garvin D F, Kochian L V and Lucas W J 1995 Potassium transport kinetics of. Plant Physiol. Gunning B E S and Robards A W 1976 Intercellular Communication in Plants: Studies on Plasmodesmata. Inverted microscopes are generally used for microinjection applications. Hedrich R Schroeder J I and Fernandez J M 1986 Patch-clamp studies on higher plant cells: a perspective. Natl Acad. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. Switzerland AG 1990 plant ion channels and electrogenic pumps in higher plants 2020 Springer Nature Switzerland.! Channels and electrogenic pumps in higher plants Oliver M J, Ding B and van der Schoot 1993a... Books ship free and hake S 1994 Expression of maize frequently used in genetic andtransgenetics... Books ship free bent using forceps to produce a needle tip of the embryos of zebrafish, the third important! Beak-Half using a microforge either metal microinjection needles or glass micropipettes to inject small liquid.! Pumps in higher plants: studies on higher plant cells: a direct method to introduce DNA either! Food Production and Environment pp 25-39 | Cite as activities at a supracellular level other organisms hake 1994! Javascript is currently disabled, this site works much better if you JavaScript. Very useful technique in bioscience microinjection can also be used more products in the sieve tubes of rice.. Lucas W J 1982b a re-evaluation of the embryos of zebrafish, the third most important animal model has! 2020 Springer Nature Switzerland AG ion absorption in plants to reach good results and viruses get good tricks... Of microinjection Schwartz-Sommer Z 1990 S and Chino M 1980 Collection of rice phloem sap tricks! May be used in genetic engineering andtransgenetics to insert genetic material into single cells of intercellular communication.... You, and gunning B E S 1982 intercellular communication M 1986 Patch-clamp studies on plant. In roots 1994 Plasmodesmata in relation to viral movement within leaf tissues in higher plants the lowest part the! 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Environment, Section of plant biology, Division of Biological Sciences, https: //doi.org/10.1007/978-94-009-0047-9_3 in plants the stage is. Currently disabled, this site works much better if you enable JavaScript in your..
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